Journal: bioRxiv

Article Title: Dual roles for influenza A protein PA-X: limiting inflammatory response and disrupting MHC I antigen presentation in human respiratory epithelium

doi: 10.64898/2026.01.30.702929

Figure Lengend Snippet: A-D) ALI cultures were infected with Perth H3N2 WT or ΔX at a multiplicity of infection (MOI) of 0.1, or mock infected and RNA was collected for scRNAseq as described in . scRNAseq data from Perth H3N2-infected ALI cultures were analyzed to identify groups of cells with similar expression patterns and to assign cell type. A) Integrated UMAP diagram of all samples, showing the 15 clusters unbiasedly identified by the program Seurat. B) Integrated UMAP diagram of all samples displaying epithelial cell types assigned with ScType. The clusters mapping to each cell type are listed in parentheses. C) Cells were collected for flow cytometric analysis at 1 DPI. (Left) Gating strategy for epithelial cell types in ALI cultures after gating for live, single cells. (Right) Stacked bar plot of average epithelial cell type composition from two different donors (Donors 4, 5). D) Alluvial plot showing the change in proportions of cells in cluster and cell type throughout infection. The two donors are plotted separately. The percentage of dead cells was calculated based on cell viability measurements using trypan blue carried out prior to dead cell depletion for scRNAseq library preparation.

Article Snippet: Data was downloaded from Trailmaker for further processing in the R program Seurat (version 5.1.0), as described by Parse Biosciences.

Techniques: Infection, Expressing